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CSP NPs inhibit VSMC calcification via downregulating NLRP3 signaling pathway. Rat vascular smooth muscle cells <t>(VSMCs)</t> (A–D), <t>human</t> <t>VSMCs</t> (E–F), or rat aortic rings (G–H) were treated with growth medium (GM), calcifying medium (CM), or CM with Nig (5 μM) in the absence or presence of CSP NPs (10 μg/mL) for 7 days (n = 4). (A) Representative alizarin red staining images of VSMCs. Scale bar = 500 μm. (B) Quantitative analysis of alizarin red staining was assessed using a microplate reader. (C–D) Runx2 and BMP2 expression was analyzed by Western blot and quantified by densitometry. (E) Representative alizarin red staining images of human VSMCs. Scale bar = 500 μm. (F) Quantitative analysis of alizarin red staining was assessed using a microplate reader. (G) Representative alizarin red staining images of rat aortic rings. Scale bar = 500 μm (low power) and 250 μm (high power). (H) Quantitative analysis of alizarin red positive area by ImageJ software. ∗ P < 0.05, ∗∗ P < 0.01.
Human Vsmcs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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smooth muscle cells line ha vsmc  (Innoprot Inc)
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CSP NPs inhibit VSMC calcification via downregulating NLRP3 signaling pathway. Rat vascular smooth muscle cells <t>(VSMCs)</t> (A–D), <t>human</t> <t>VSMCs</t> (E–F), or rat aortic rings (G–H) were treated with growth medium (GM), calcifying medium (CM), or CM with Nig (5 μM) in the absence or presence of CSP NPs (10 μg/mL) for 7 days (n = 4). (A) Representative alizarin red staining images of VSMCs. Scale bar = 500 μm. (B) Quantitative analysis of alizarin red staining was assessed using a microplate reader. (C–D) Runx2 and BMP2 expression was analyzed by Western blot and quantified by densitometry. (E) Representative alizarin red staining images of human VSMCs. Scale bar = 500 μm. (F) Quantitative analysis of alizarin red staining was assessed using a microplate reader. (G) Representative alizarin red staining images of rat aortic rings. Scale bar = 500 μm (low power) and 250 μm (high power). (H) Quantitative analysis of alizarin red positive area by ImageJ software. ∗ P < 0.05, ∗∗ P < 0.01.
Smooth Muscle Cells Line Ha Vsmc, supplied by Innoprot Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vascular smooth muscle cells vsmcs  (PromoCell)
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CSP NPs inhibit VSMC calcification via downregulating NLRP3 signaling pathway. Rat vascular smooth muscle cells <t>(VSMCs)</t> (A–D), <t>human</t> <t>VSMCs</t> (E–F), or rat aortic rings (G–H) were treated with growth medium (GM), calcifying medium (CM), or CM with Nig (5 μM) in the absence or presence of CSP NPs (10 μg/mL) for 7 days (n = 4). (A) Representative alizarin red staining images of VSMCs. Scale bar = 500 μm. (B) Quantitative analysis of alizarin red staining was assessed using a microplate reader. (C–D) Runx2 and BMP2 expression was analyzed by Western blot and quantified by densitometry. (E) Representative alizarin red staining images of human VSMCs. Scale bar = 500 μm. (F) Quantitative analysis of alizarin red staining was assessed using a microplate reader. (G) Representative alizarin red staining images of rat aortic rings. Scale bar = 500 μm (low power) and 250 μm (high power). (H) Quantitative analysis of alizarin red positive area by ImageJ software. ∗ P < 0.05, ∗∗ P < 0.01.
Vascular Smooth Muscle Cells Vsmcs, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human t g ha vsmc cell line  (ATCC)
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CSP NPs inhibit VSMC calcification via downregulating NLRP3 signaling pathway. Rat vascular smooth muscle cells <t>(VSMCs)</t> (A–D), <t>human</t> <t>VSMCs</t> (E–F), or rat aortic rings (G–H) were treated with growth medium (GM), calcifying medium (CM), or CM with Nig (5 μM) in the absence or presence of CSP NPs (10 μg/mL) for 7 days (n = 4). (A) Representative alizarin red staining images of VSMCs. Scale bar = 500 μm. (B) Quantitative analysis of alizarin red staining was assessed using a microplate reader. (C–D) Runx2 and BMP2 expression was analyzed by Western blot and quantified by densitometry. (E) Representative alizarin red staining images of human VSMCs. Scale bar = 500 μm. (F) Quantitative analysis of alizarin red staining was assessed using a microplate reader. (G) Representative alizarin red staining images of rat aortic rings. Scale bar = 500 μm (low power) and 250 μm (high power). (H) Quantitative analysis of alizarin red positive area by ImageJ software. ∗ P < 0.05, ∗∗ P < 0.01.
Human T G Ha Vsmc Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human primary aortic vascular smooth muscle cells  (ATCC)
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ATCC human primary aortic vascular smooth muscle cells
CSP NPs inhibit VSMC calcification via downregulating NLRP3 signaling pathway. Rat vascular smooth muscle cells <t>(VSMCs)</t> (A–D), <t>human</t> <t>VSMCs</t> (E–F), or rat aortic rings (G–H) were treated with growth medium (GM), calcifying medium (CM), or CM with Nig (5 μM) in the absence or presence of CSP NPs (10 μg/mL) for 7 days (n = 4). (A) Representative alizarin red staining images of VSMCs. Scale bar = 500 μm. (B) Quantitative analysis of alizarin red staining was assessed using a microplate reader. (C–D) Runx2 and BMP2 expression was analyzed by Western blot and quantified by densitometry. (E) Representative alizarin red staining images of human VSMCs. Scale bar = 500 μm. (F) Quantitative analysis of alizarin red staining was assessed using a microplate reader. (G) Representative alizarin red staining images of rat aortic rings. Scale bar = 500 μm (low power) and 250 μm (high power). (H) Quantitative analysis of alizarin red positive area by ImageJ software. ∗ P < 0.05, ∗∗ P < 0.01.
Human Primary Aortic Vascular Smooth Muscle Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human vascular smooth muscle cells  (ATCC)
96
ATCC human vascular smooth muscle cells
CSP NPs inhibit VSMC calcification via downregulating NLRP3 signaling pathway. Rat vascular smooth muscle cells <t>(VSMCs)</t> (A–D), <t>human</t> <t>VSMCs</t> (E–F), or rat aortic rings (G–H) were treated with growth medium (GM), calcifying medium (CM), or CM with Nig (5 μM) in the absence or presence of CSP NPs (10 μg/mL) for 7 days (n = 4). (A) Representative alizarin red staining images of VSMCs. Scale bar = 500 μm. (B) Quantitative analysis of alizarin red staining was assessed using a microplate reader. (C–D) Runx2 and BMP2 expression was analyzed by Western blot and quantified by densitometry. (E) Representative alizarin red staining images of human VSMCs. Scale bar = 500 μm. (F) Quantitative analysis of alizarin red staining was assessed using a microplate reader. (G) Representative alizarin red staining images of rat aortic rings. Scale bar = 500 μm (low power) and 250 μm (high power). (H) Quantitative analysis of alizarin red positive area by ImageJ software. ∗ P < 0.05, ∗∗ P < 0.01.
Human Vascular Smooth Muscle Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary vascular smooth muscle cells  (Cell Applications Inc)
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Cell Applications Inc primary vascular smooth muscle cells
CSP NPs inhibit VSMC calcification via downregulating NLRP3 signaling pathway. Rat vascular smooth muscle cells <t>(VSMCs)</t> (A–D), <t>human</t> <t>VSMCs</t> (E–F), or rat aortic rings (G–H) were treated with growth medium (GM), calcifying medium (CM), or CM with Nig (5 μM) in the absence or presence of CSP NPs (10 μg/mL) for 7 days (n = 4). (A) Representative alizarin red staining images of VSMCs. Scale bar = 500 μm. (B) Quantitative analysis of alizarin red staining was assessed using a microplate reader. (C–D) Runx2 and BMP2 expression was analyzed by Western blot and quantified by densitometry. (E) Representative alizarin red staining images of human VSMCs. Scale bar = 500 μm. (F) Quantitative analysis of alizarin red staining was assessed using a microplate reader. (G) Representative alizarin red staining images of rat aortic rings. Scale bar = 500 μm (low power) and 250 μm (high power). (H) Quantitative analysis of alizarin red positive area by ImageJ software. ∗ P < 0.05, ∗∗ P < 0.01.
Primary Vascular Smooth Muscle Cells, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CSP NPs inhibit VSMC calcification via downregulating NLRP3 signaling pathway. Rat vascular smooth muscle cells (VSMCs) (A–D), human VSMCs (E–F), or rat aortic rings (G–H) were treated with growth medium (GM), calcifying medium (CM), or CM with Nig (5 μM) in the absence or presence of CSP NPs (10 μg/mL) for 7 days (n = 4). (A) Representative alizarin red staining images of VSMCs. Scale bar = 500 μm. (B) Quantitative analysis of alizarin red staining was assessed using a microplate reader. (C–D) Runx2 and BMP2 expression was analyzed by Western blot and quantified by densitometry. (E) Representative alizarin red staining images of human VSMCs. Scale bar = 500 μm. (F) Quantitative analysis of alizarin red staining was assessed using a microplate reader. (G) Representative alizarin red staining images of rat aortic rings. Scale bar = 500 μm (low power) and 250 μm (high power). (H) Quantitative analysis of alizarin red positive area by ImageJ software. ∗ P < 0.05, ∗∗ P < 0.01.

Journal: Redox Biology

Article Title: Ultrasmall Cu 2−x Se nanoparticles alleviate vascular calcification through inhibiting oxidative stress and NF-κB/NLRP3-mediated inflammation

doi: 10.1016/j.redox.2025.103961

Figure Lengend Snippet: CSP NPs inhibit VSMC calcification via downregulating NLRP3 signaling pathway. Rat vascular smooth muscle cells (VSMCs) (A–D), human VSMCs (E–F), or rat aortic rings (G–H) were treated with growth medium (GM), calcifying medium (CM), or CM with Nig (5 μM) in the absence or presence of CSP NPs (10 μg/mL) for 7 days (n = 4). (A) Representative alizarin red staining images of VSMCs. Scale bar = 500 μm. (B) Quantitative analysis of alizarin red staining was assessed using a microplate reader. (C–D) Runx2 and BMP2 expression was analyzed by Western blot and quantified by densitometry. (E) Representative alizarin red staining images of human VSMCs. Scale bar = 500 μm. (F) Quantitative analysis of alizarin red staining was assessed using a microplate reader. (G) Representative alizarin red staining images of rat aortic rings. Scale bar = 500 μm (low power) and 250 μm (high power). (H) Quantitative analysis of alizarin red positive area by ImageJ software. ∗ P < 0.05, ∗∗ P < 0.01.

Article Snippet: Human VSMCs (CRL-1999, ATCC, USA) were cultured under identical conditions.

Techniques: Staining, Expressing, Western Blot, Software